JT AFC HC 2024

Thursday 13 June 2024 - Institut Pasteur, Lille, France

Bandeau - JT AFC HC 2024

Thursday 13 June

Stairway to High-Content - Part 1 - Suspension Cytometry

07:30-08:00

Registration


08:00-08:30

Welcoming


08:30-09:00

Introduction to High-Content Cytometry

Introduction


Theory of High-Content

  • Spectral
  • Mass
  • Data analysis

09:00-10:30

HC Cytometry brain diseases

  • Unlocking novel brain pathways and neuronal functions: Groundbreaking discoveries through FACS Isolation
    Pr. Vincent Prévot - LilNCog, Lille
  • Télécharger le PDF A brain-to-skin axis orchestrating tissue-resident macrophages healing properties
    Dr. Guillaume Hoeffel - CIML, Marseille
  • Télécharger le PDF Compartmentalization of myeloid cell responses in neuroinflammation
    Dr. Chotima Böttcher - Charité (ECRC), Berlin, Germany

10:30-11:00

Coffee break


11:00-12:30

Data analysis of HC Cytometry

  • Data QC tips for analysis of massive parallel cytometry approaches
    Dr. Hervé Luche - Ciphe, Marseille
  • Télécharger le PDF From algorithms to applications
    Dr. Sofie Van Gassen - VIB/UGent, Ghent, Belgium
  • Télécharger le PDF Standardized high-dimensional spectral cytometry protocol and panels for whole blood immune phenotyping in clinical and translational studies
    Dr. Slobodan Culina - Institut Pasteur, Paris

12:30-13:30

Lunch Box


13:30-14:30

Round table Data analysis

Pipeline/Normalisation


Dim reduction/clustering


14:30-16:30

Round table Mass/Spectral

Sample managment


Stainings and protocoles


Panel design


16:30

Coffee and bye bye’s