Thursday 13 June
Stairway to High-Content - Part 1 - Suspension Cytometry
07:30-08:00
Registration
08:00-08:30
Welcoming
08:30-09:00
Introduction to High-Content Cytometry
Introduction
Theory of High-Content
- Spectral
- Mass
- Data analysis
09:00-10:30
HC Cytometry brain diseases
- Unlocking novel brain pathways and neuronal functions: Groundbreaking discoveries through FACS Isolation
Pr. Vincent Prévot - LilNCog, Lille - A brain-to-skin axis orchestrating tissue-resident macrophages healing properties
Dr. Guillaume Hoeffel - CIML, Marseille - Compartmentalization of myeloid cell responses in neuroinflammation
Dr. Chotima Böttcher - Charité (ECRC), Berlin, Germany
10:30-11:00
Coffee break
11:00-12:30
Data analysis of HC Cytometry
- Data QC tips for analysis of massive parallel cytometry approaches
Dr. Hervé Luche - Ciphe, Marseille - From algorithms to applications
Dr. Sofie Van Gassen - VIB/UGent, Ghent, Belgium - Standardized high-dimensional spectral cytometry protocol and panels for whole blood immune phenotyping in clinical and translational studies
Dr. Slobodan Culina - Institut Pasteur, Paris
12:30-13:30
Lunch Box
13:30-14:30
Round table Data analysis
Pipeline/Normalisation
Dim reduction/clustering
14:30-16:30
Round table Mass/Spectral
Sample managment
Stainings and protocoles
Panel design
16:30