Atelier 4
Workshop on flow cytometry measurement and analyses of signaling in primary cell subsets
J. Irish (Nashville) C. Bezombes (UMR1037, Toulouse), E. Decaup (UMR1037, Toulouse)
Cell signaling normally governs checkpoints throughout development, and alteration of signaling plays a driving role in human diseases, such as cancer.
Flow cytometry allows high-content, multiparameter analysis of single cells, especially useful in comparing subsets of cells present within heterogeneous primary tissue samples, making it a promising tool for drug discovery and profiling of intracellular signalling.
We will present the fluorescent cell bar coding developped in Stanford by G Nolan’s group, which is a cell-based mutliplexing technique allowing a high-throughput and saving time and fees. Analyses will be performed with Cytobank, a Web-based application for storage, analysis, and sharing of flow cytometry experiments.